Evaluation of metabolome sample preparation and extraction methodologies for oleaginous filamentous fungi Mortierella alpina

ConclusionThe use of freeze-dried biomass for metabolite extraction and data normalisation was more efficient at measuring the active state of the intracellular metabolites inM. alpina. We recommend extracting the intracellular metabolites with methanol:water (1:1). An important role of amino acid oxidation in the nitrogen limitation-mediated lipid accumulation was found.

Source: Metabolomics - March 20, 2019 Category: Biology Source Type: research