Cationic lipoplexes for treatment of cancer stem cell-derived murine lung tumors

This study outlines the fabrication of a lipoplex of SOX2 small interfering RNA (CL-siSOX2) for targeted treatment of SOX2-enriched, CSC-derived orthotopic and xenograft lung tumors in CB-17 SCID mice. CL-siSOX2 induced tumor contraction in cisplatin-naïve and cisplatin-treated groups by 85% and 94% respectively. Reduction in tumor weight and volume following treatment with CL-siSOX2 was associated with reduced protein expression of SOX2 and markers of tumor initiation, inflammation, invasion and metastasis in mice tumor xenografts. In addition, histological staining of lung tumor sections showed reduction in SOX2 expression was associated with inhibition markers of epithelial-to-mesenchymal transition.Graphical AbstractSchematic showing clonal expansion of H1650 side population (SP) cells and modeling of murine lung tumor in SCID-beige mice. Mice were administered SOX2 siRNA-containing cationic lipoplexes alone or in combination with cisplatin resulting in reduction of tumor volume.
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research