Development and in vitro characterization of an oral self-emulsifying delivery system (SEDDS) for rutin fatty ester with high mucus permeating properties

Publication date: Available online 18 March 2019Source: International Journal of PharmaceuticsAuthor(s): Maria I. Cardona, Nguyet-Minh Nguyen Le, Sergey Zaichik, Diana M. Aragón, Andreas Bernkop- SchnürchAbstractThe aim of this study was to develop and evaluate a self-emulsifying delivery system (SEDDS) for oral rutin fatty ester administration and to improve its mucus permeating properties by the incorporation of the silicon polymer poly [dimethylsiloxane-co-(3-(2-(2-hydroxyethoxy)ethoxy)propyl]methylsiloxane] (PDMSHEPMS) in the formulation. In order to increase the lipophilicity of the flavonoid and to dissolve it in SEDDS, enzymatic acylation of rutin with lauric acid was catalyzed by lipase from Candida antarctica in acetone. Different formulations were evaluated regarding their emulsifying properties and ability to dissolve the rutin ester. Suitable SEDDS was chosen and characterized regarding droplet size, polydispersity index, and zeta potential. The rutin fatty ester was loaded into SEDDS to 7% (w/w). Different concentrations of PDMSHEPMS were incorporated in SEDDS for following mucus permeation studies. Formulation with 10% of PDMSHEPMS showed 1.9-fold increase in mucus permeation compared to the formulation without PDMSHEPMS. Furthermore, the formulation with 10% of PDMSHEPMS showed a significant increase in mucus permeation compared with rutin fatty ester without formulation. According to these results, SEDDS containing PDMSHEPMS might be a promising st...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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The surface interactions between gold nanoparticles (AuNPs) and lipase from Candida antarctica fraction B (CALB) were investigated at macromolecular and colloidal length-scales. In order to elucidate the reciprocal effect of the interactions on the individual component ’ s properties, CALB was either added during the synthesis of AuNPs or added to pre-synthesized AuNPs. In both cases, it was observed that the AuNPs are spherical and stable and, by fluorescence and circular dichroism spectroscopies, changes were observed in the secondary and tertiary structure of CALB, that were dependent on the AuNPs concentration. N...
Source: Journal of the Brazilian Chemical Society - Category: Chemistry Source Type: research
Genes, Vol. 10, Pages 831: The Mechanisms of Mating in Pathogenic Fungi—A Plastic Trait Genes doi: 10.3390/genes10100831 Authors: Jane Usher The impact of fungi on human and plant health is an ever-increasing issue. Recent studies have estimated that human fungal infections result in an excess of one million deaths per year and plant fungal infections resulting in the loss of crop yields worth approximately 200 million per annum. Sexual reproduction in these economically important fungi has evolved in response to the environmental stresses encountered by the pathogens as a method to target DNA damage. Meiosis...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Review Source Type: research
Publication date: Available online 19 October 2019Source: Inorganica Chimica ActaAuthor(s): Ayman K. El-Sawaf, El Hassane AnouarAbstractThe (E)-N'-(2-hydroxybenzylidene)morpholine-4-carbothiohydrazide (H2L) ligand and its chelates with Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) have been synthesized. Analytical and spectral data prove that the ligand acts a monobasic tridentate ligand coordinated via deprotonated phenolic O, azomethine N and thione S atoms (ONS chromophore) with cobalt(II), nickel(II) and copper(II) salts leading to two fused five and six-membered chelate rings. While with zinc(II) and cadmium(II) salts the...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
Publication date: Available online 18 October 2019Source: Microbiological ResearchAuthor(s): Marina Zoppo, Mariagrazia Di Luca, Mauro Franco, Cosmeri Rizzato, Antonella Lupetti, Annarita Stringaro, Flavia De Bernardis, Christoph Schaudinn, M. Inmaculada Barrasa, Daria Bottai, Valmik K. Vyas, Arianna TavantiAbstractThe ability of yeast to adhere to biotic and abiotic surfaces represents an essential trait during the early stages of infection. Agglutinin-like sequence (Als) cell-wall proteins play a key role in adhesion of Candida species. Candida parapsilosis genome encompasses 5 ALS members, of which only the role of CPAR2...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research
Publication date: 5 February 2020Source: Journal of Molecular Structure, Volume 1201Author(s): Rasha A. Azzam, Galal H. Elgemeie, Rokia R. OsmanAbstractThe synthesis of novel pyrido[2,1-b]benzothiazole and pyrido[2,1-b]benzoimidazole derivatives was achieved via the reaction of N-aryl-2-cyano-3,3-bis(methylthio)acrylamide with benzothiazoleylacetonitrile and benzoimidazoleylacetonitrile, respectively, while N-substituted 2-pyridylbenzothiazole derivatives were synthesized by reacting 2-(benzo[d]thiazol-2-yl)-3-(dimethylamino)acrylonitrile with either cyanoacetamide, aryl cyanoacetamides or 2-cyano-N’-(4-substituted b...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
ConclusionWe found few genetically-related as well as identical isolates among the 32 Candida strains which indicated low cross-contaminations among the patients. There was no relationship between C. albicans MLST profiles and their geographic distribution, cancer type and SAP2 gene expression. This lack of correlation was possibly due to the small understudy population; hence, finding more relevance requires studies with a higher number of samples.
Source: Journal of Medical Mycology - Category: Biology Source Type: research
This study explored Candida antarctica lipase B (CALB) non-covalently immobilized on multiwalled carbon nanotubes for the preparation of metal-free (co)polymers ofε-caprolactone (CL) and trimethylene carbonate (TMC). The novel initiating/catalytic system based on nanobiocatalyst and 1,5-pentanediol allows for the control over the molar mass (Mn) of the obtained homopolymers and copolymers. Mn of polymers, ranged from 5.0 to 50 kg/mol, was controlled by monomer to initiator ratio. Their microstructure was investigated by 13C NMR, while thermal properties by DSC. In addition, the recycling studies indicate that nan...
Source: Polymer Degradation and Stability - Category: Chemistry Source Type: research
The objective of this study was to evaluate the toxicity of the antiparasitic agent eprinomectin in two subtropical soils, using ecotoxicological lethality, reproduction and avoidance behavior tests with springtails (Folsomia candida). Eprinomectin concentrations were 0 (control), 0.5, 1, 2, 4, 8, 12, 16 and 20 mg kg−1 of dry soil combined with either Entisol or Oxisol soils. Statistically significant toxic effects of eprinomectin on springtails were observed in both soils. Eprinomectin was lethal starting at 8 mg kg-1 of dry soil in Entisol, and 20 mg kg−1 of dry soil in Oxisol, with effects less t...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research
Abstract Candida albicans is the most common clinical pathogenic fungus, which is highly susceptible to immunodeficiency. Development of novel antifungal agents has become a growing trend in the treatment of Candida infections. C16-Fengycin A, a lipopeptide isolated from Bacillus amyloliquefaciens fmb60 showed significant fungicidal activity against C. albicans. In the study, we explored the possible antifungal mode of C16-Fengycin A. It was predicted that C16-Fengycin A had the ability to disrupt the cell wall due to its alterations of cell ultrastructure, and reduction of cell wall hydrophobicity. This was furth...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
In this study, we show that highly immunogenic C. albicans hyphae attract phagocytic cells, which rapidly engulf adherent S. aureus and subsequently migrate to cervical lymph nodes. Following S. aureus-loaded phagocyte translocation from the mucosal surface, S. aureus produces systemic disease with accompanying morbidity and mortality. Our results suggest a novel role for the host in facilitating a bacterium-fungus infectious synergy, leading to disseminated staphylococcal disease.
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Host-Associated Microbial Communities Source Type: research
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