Cellular immune response and scanning electron microscopy in the evaluation of Moringa leaves aqueous extract effect on Cryptosporidium parvum in buffalo intestinal tissue explants

In this study, an explant culture was used  as an in vitro model of buffalo intestine to evaluate the effect of Moringa leaves extract onCryptosporidium parvum (C. parvum) oocysts using light and scanning electron microscopy and measuring IFN- γ, IL-12 and IL-14 in the culture supernatants.C. parvum oocysts were collected from naturally-infected calf feces, isolated, excysted and then co-inoculated with ileal tissue explants culture medium. The prepared Moringa leaves extract was then introduced to the infected tissues in the concentrations of 100  mg/ml and 300 mg/ml. After 24 h, tissues were collected and processed for light and scanning electron microscopy. Also, culture supernatants were collected for cytokines measurement.C. parvum parasitophorous vacuoles were found attached to the surface of tissue inCryptosporidium-infected ileal tissue explants. High magnification imaging of ileal tissue explants using scanning electron microscopy showed that Moringa leaves extracts had a great effect onCryptosporidium-infected ileal tissue explants. There was a high significant (P  <  0.001) increase in IFN-γ, IL-12 and IL-14 (375, 275 and 90 pg/ml, respectively) in the supernatants of infected non-treated ileal tissue explant culture plate wells compared to the control non-infected ones (74.66, 75 and 50 pg/ml, respectively). A concentration of 100 mg/ml Moringa extract e xhibited the highest anticryptosporidial effect causing a significant decrease in IFN-γ, IL-12...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research