A SERS nano-tag-based magnetic-separation strategy for highly sensitive immunoassay in unprocessed whole blood.

A SERS nano-tag-based magnetic-separation strategy for highly sensitive immunoassay in unprocessed whole blood. Talanta. 2019 Jun 01;198:527-533 Authors: Zhao P, Li HX, Li DW, Hou YJ, Mao L, Yang M, Wang Y Abstract Assay technologies capable of detecting biomarker concentrations in unprocessed whole blood samples are fundamental for applications in medical diagnostics. SERS nano-tags integrated magnetic-separation biosensor (MSB) was realized for the first time for immunoassay in whole blood. The reliability and sensitivity of this method rely, in a large extent, on the quality and properties of the SERS nano-tags. The constructed silicacoated Ag SERS nano-tags as labels were used in a rapid and specific MSB immune sensor to detect Matrix Metalloproteinases 9 (MMP-9) in unprocessed blood samples. With fast screening ability and outstanding sensitivity, we anticipate that this method would greatly promote practical application of stroke-based early-stage cancer diagnosis. PMID: 30876595 [PubMed - in process]
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research