The protective role of Piriformospora indica colonization in Centella asiatica (L.) in vitro under phosphate stress

Publication date: Available online 18 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): S. Jisha, K.N. Anith, K.K. SabuAbstractThe core of present study was to determine potential role of Piriformospora indica, mutualistic Basidiomycete endosymbiont on co-cultivation with Centella asiatica (L.) Urb., a neutraceutical plant. The analysis of major secondary metabolite asiaticoside and differential expression of key genes involved in pathway under varying phosphate (P) concentrations maintained in vitro were carried out. Maximum asiaticoside content was documented in plants maintained in MS medium containing lowest level (62.5 μM) tried (p < 0.001). At phosphate concentration of 125 μ M (10% of normal P), significant stimulation of plant growth and asiaticoside production were observed in colonized plants. P levels at 125 μM however favored maximum fresh weight, root and shoot length, root and leaf number of plants. The transcript accumulation of β Amyrin Synthase, the key gene in asiaticoside pathway was recorded at the P level of 62.5 μM, which is significantly higher (p < 0.001) than those at other P concentrations tried which was evidenced by overproduction of auxin, Indole-3-Acetic Acid (IAA). A significantly higher level of IAA was recorded in P. indica colonized plants maintained in low (125 μM) P medium. The presence of P. indica under low phosphate concentrations has protective role in mitigating effects of stress, as ...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research