The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: a pilot study

Publication date: Available online 17 March 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Božena Novotná, Jitka Sikorová, Alena Milcová, Martin Pechout, Luboš Dittrich, Michal Vojtíšek-Lom, Pavel Rossner, Táňa Brzicová, Jan TopinkaAbstractAn analysis of the toxic effects of emissions should reflect real traffic conditions. The exhaust emissions of particulate matter from diesel engines strongly depend on their operating conditions, with low-speed, low-load "urban creep" conditions, common for truck traffic in heavily congested urban areas, being one of the worst. We aimed to detect the genotoxicity of organic extracts from particulate matter in the exhaust of the diesel engine Zetor 1505 running on diesel and biodiesel (B100) fuels at characteristic modes of extended "urban creep", typical for transit truck traffic in Prague, comparing the first 5 minutes of idling with extended (20-80 minute) idling, full load after idle, "stabilized" full load, and 30 % load. The diluted exhaust was sampled with high volume samplers on glass fiber fluorocarbon coated filters. The filters were extracted with dichloromethane and DNA damage was analyzed in A549 cells using comet assay, with the inclusion of formamidopyrimidine DNA glycosylase (FPG) and endonuclease III (ENDOIII) to recognize oxidized DNA bases. The cells were exposed to extractable organic matter (EOM) for 4 and 24 hours at non-cytotoxic dose corresponding to 0.001 m3 ...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research