Optimization of media components for the production of N-acetylchitooligosaccharide from chitin by Streptomyces chilikensis through Taguchi experimental design

Publication date: Available online 16 March 2019Source: Journal of Microbiological MethodsAuthor(s): Himadri Tanaya Behera, Anjani Kumar Upadhyay, Vishakha Raina, Lopamudra RayAbstractOptimization of media composition for microbial growth is crucial particularly in industrial processes to obtain the desired end product. The waste from sea food industries includes the non-edible parts of shrimp, crabs and prawns which are rich in chitin as the major cause of pollution in coastal areas. Chitin degradation is carried out chemically. It can be degraded biologically also, particularly using microorganisms resulting in chitooligosaccahrides and the monomer N-acetylglucosamine. N-acetyl glucosamine and related chitooligosaccahrides have various applications such as treatment of cancer and metastasis, treatment of autoimmune reactions, as food supplements and increased plant stress tolerance against salinity and heavy metals. Thus, chitin waste can be efficiently degraded biologically using microorganisms to produce such useful products. Conventional methods such as One factor at a time (OFAT) are more time consuming and costly to address the problem. The current work focuses on the development of an experimental design to ascertain parameters optimized for chitin degradation by a Streptomyces chilikensis to produce various chitooligosaccharides. More than one factor was taken at a time to carry out the experiments and the data were fit into Taguchi Design to determine the contributi...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research