A Practical Guide to Genome Editing Using Targeted Nuclease Technologies.

A Practical Guide to Genome Editing Using Targeted Nuclease Technologies. Compr Physiol. 2019 Mar 14;9(2):665-714 Authors: Porter SN, Levine RM, Pruett-Miller SM Abstract Genome engineering using programmable nucleases is a rapidly evolving technique that enables precise genetic manipulations within complex genomes. Although this technology first surfaced with the creation of meganucleases, zinc finger nucleases, and transcription activator-like effector nucleases, CRISPR-Cas9 has been the most widely adopted platform because of its ease of use. This comprehensive review presents a basic overview of genome engineering and discusses the major technological advances in the field. In addition to nucleases, we discuss CRISPR-derived base editors and epigenetic modifiers. We also delve into practical applications of these tools, including creating custom-edited cell and animal models as well as performing genetic screens. Finally, we discuss the potential for therapeutic applications and ethical considerations related to employing this technology in humans. © 2019 American Physiological Society. Compr Physiol 9:665-714, 2019. PMID: 30873595 [PubMed - in process]
Source: Comprehensive Physiology - Category: Physiology Tags: Compr Physiol Source Type: research