Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Publication date: Available online 16 March 2019Source: Stem Cell ResearchAuthor(s): Samuel McLenachan, Elaine Y.M. Wong, Xiao Zhang, Fiona Leith, Sang Yoon Moon, Dan Zhang, Shang-Chih Chen, Jennifer A. Thompson, Terri McLaren, Tina Lamey, John N. De Roach, Marcus D. Atlas, Rodney J. Dilley, Fred K. ChenAbstractThe human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.
Source: Stem Cell Research - Category: Stem Cells Source Type: research