Viruses, Vol. 11, Pages 259: Generation and Characterization of Anti-Filovirus Nucleoprotein Monoclonal Antibodies

Viruses, Vol. 11, Pages 259: Generation and Characterization of Anti-Filovirus Nucleoprotein Monoclonal Antibodies Viruses doi: 10.3390/v11030259 Authors: Md Niaz Rahim Min Wang Tong Wang Shihua He Bryan D. Griffin Darwyn Kobasa Ruifu Yang Zongmin Du Xiangguo Qiu Filoviruses cause lethal hemorrhagic fever in humans. The filovirus nucleoprotein (NP) is expressed in high abundance in infected cells and is essential for virus replication. To generate anti-filovirus monoclonal antibodies (mAbs) against the NP, mice were immunized with peptides known as B-cell epitopes corresponding to different filovirus NPs, and hybridomas were screened using FLAG-tagged filovirus NP constructs. Numerous mAbs were identified, isotyped, and characterized. The anti-NP mAbs demonstrated different ranges of binding affinities to various filovirus NPs. Most of the clones specifically detected both recombinant and wild-type NPs from different filoviruses, including Ebola (EBOV), Sudan (SUDV), Bundibugyo (BDBV), Marburg (MARV), Tai Forest (TAFV), and Reston (RESTV) viruses in western blot analysis. The mAbs were also able to detect native NPs within the cytoplasm of infected cells by immunofluorescence confocal microscopy. Thus, this panel of mAbs represents an important set of tools that may be potentially useful for diagnosing filovirus infection, characterizing virus replication, and detecting NP–host protein interactions.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research