BacMam production and crystal structure of nonglycosylated apo human furin at 1.89   Å resolution

Furin, also called proprotein convertase subtilisin/kexin 3 (PCSK3), is a calcium-dependent serine endoprotease that processes a wide variety of proproteins involved in cell function and homeostasis. Dysregulation of furin has been implicated in numerous disease states, including cancer and fibrosis. Mammalian cell expression of the furin ectodomain typically produces a highly glycosylated, heterogeneous protein, which can make crystallographic studies difficult. Here, the expression and purification of nonglycosylated human furin using the BacMam technology and site-directed mutagenesis of the glycosylation sites is reported. Nonglycosylated furin produced using this system retains full proteolytic activity indistinguishable from that of the glycosylated protein. Importantly, the nonglycosylated furin protein reliably forms extremely durable apo crystals that diffract to high resolution. These crystals can be soaked with a wide variety of inhibitors to enable a structure-guided drug-discovery campaign.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: furin nonglycosylated apo serine proteinase hydrolase BacMam research communications Source Type: research

Related Links:

Publication date: Available online 4 April 2020Source: Journal of Geriatric OncologyAuthor(s): Kelly M. Trevino, Amy Stern, Holly G. Prigerson
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 4 April 2020Source: Journal of Geriatric OncologyAuthor(s): Claire A. Surr, Rachael Kelley, Alys W. Griffiths, Laura Ashley, Fiona Cowdell, Ann Henry, Michelle Collinson, Ellen Mason, Amanda J. Farrin
Source: Journal of Geriatric Oncology - Category: Cancer & Oncology Source Type: research
Abstract OBJECTIVES: To identify and discuss scientific evidence of the effects of ginger use on the management of chemotherapy-induced nausea and vomiting. METHODS: This is an integrative reviewperformed by Ganong's reference. RESULTS: We included 24 studies, highlighting three thematic categories, namely 1) antiemetic action of ginger - nausea (13 articles; of these, nine significant) and emesis (10 studies; of these, six significant); 2) action in the control of nausea (11 articles; of these, six significant) and vomiting (8 articles; of these, three significant) in the acute phase; 3) action in the c...
Source: Revista Brasileira de Enfermagem - Category: Nursing Authors: Tags: Rev Bras Enferm Source Type: research
Connection between SOX7 Expression and Breast Cancer Prognosis. Med Sci Monit. 2020 Apr 02;26:e921510 Authors: Qin CX, Yang XQ, Zhan ZY Abstract BACKGROUND SOX7 exerts a repressing effect against tumors and imposes vital influences on malignancies. Our research discussed the importance of SOX7 in breast cancer prognoses. MATERIAL AND METHODS SOX7 mRNA expression in breast cancer tissues samples and matched adjacent normal controls of breast cancer patients was measured by quantitative real-time-polymerase chain reaction (qRT-PCR). The relationship of SOX7 with clinicopathological characteristics w...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
CONCLUSIONS These 7 DEGs might be potential diagnostic and prognostic biomarkers for PAC and help uncovering the mechanism of PAC. PMID: 32235821 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Publication date: Available online 3 April 2020Source: Food BioscienceAuthor(s): M.S. Taniya, Reshma MV, Shanimol PS, Gayatri Krishnan, Priya S
Source: Food Bioscience - Category: Food Science Source Type: research
CONCLUSIONS: Gasless r-PRP is an efficient and safe method in prostate cancer surgery. However, prospective randomized and comparative studies are required with large patient series. PMID: 32240115 [PubMed - in process]
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
In this study, we aimed to evaluate whether the presence of TM has an effect on GCTC prognosis and oncological outcomes. METHODS: Seventy five patients among 93 patients who underwent radical orchidectomy between January 2010 and February 2016 were selected and divided into two groups. Group I consisted of 51 patients without TM. Group II consisted of 24 patients with TM. Each groups were compared in terms of demographic datas, prognostic risk factors, complete blood count parameters and oncological outcomes. RESULTS: During the median follow-up of 58 (1-106) months, a significantly higher local recurrence rate (54.2% vs. 3.9%, p
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
CONCLUSION: In our study population, patients  ≤55 years treated with RRP did not present differences in the pathologic features of prostate cancer or in biochemical recurrence rates in comparison to the group of older patients. PMID: 32240111 [PubMed - in process]
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
Authors: Bonet X, Suarez-Novo JF, Castells M, Serrallach M, Beato S, Picola N, Boladeras A, Ferrer F, Slocker A, Merino EM, García-Benet JR, Condom E, Hajianfar R, Heras L, Piulats JM, Vigués F Abstract OBJECTIVE: To describe the initial experiencein our center on targeted prostate biopsies (TB) using Magnetic Resonance imaging/ultrasonography (MRI/US) fusion and to compare PCa detection with systematic biopsies (SB). PATIENTS AND ME THODS: A retrospective, descriptive and comparative study was conducted on the first 94 men who underwent TB using MRU/US fusion in our center since February 2017 to ...
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
More News: Biochemistry | Calcium | Cancer | Cancer & Oncology | Study