The Pleiotropic Effects of c-di-GMP Content in Pseudomonas syringae.

The Pleiotropic Effects of c-di-GMP Content in Pseudomonas syringae. Appl Environ Microbiol. 2019 Mar 08;: Authors: Wang T, Cai Z, Shao X, Zhang W, Xie Y, Zhang Y, Hua C, Schuster SC, Yang L, Deng X Abstract Although the ubiquitous bacterial secondary messenger cyclic diguanylate (c-di-GMP) has important cellular functions in a wide range of bacteria, its function in the model plant pathogen Pseudomonas syringae (P. syringae) remains largely elusive. To this end, we overexpressed Escherichia coli diguanylate cyclase (YedQ) or phosphodiesterase (YhjH) in P. syringae, resulting in high or low in vivo levels of c-di-GMP. Via genome-wide RNA sequencing of these two strains, we found that c-di-GMP regulates (i) fliN, fliE, and flhA, which are associated with flagellar assembly; (ii) alg8 and alg44, which are related to the exopolysaccharide biosynthesis pathway; (iii) pvdE, pvdP, and pvsA, which are associated with the siderophore biosynthesis pathway; and (iv) sodA, which encodes a superoxide dismutase. In particular, we identified three promoters that are sensitive to elevated levels of c-di-GMP and inserted them into luciferase-based reporters that respond effectively to the c-di-GMP levels in P. syringae; these promoters could be useful in the measurement of in vivo levels of c-di-GMP in real time. Further phenotypic assays validated the RNA-seq results and confirmed the effect on c-di-GMP-associated pathways, such as repressing the t...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research