Effect of short-term sterigmatocystin exposure on lipid peroxidation and glutathione redox system and expression of glutathione redox system regulatory genes in common carp liver.

Effect of short-term sterigmatocystin exposure on lipid peroxidation and glutathione redox system and expression of glutathione redox system regulatory genes in common carp liver. Toxicon. 2019 Mar 05;: Authors: Kövesi B, Pelyhe C, Zándoki E, Mézes M, Balogh K Abstract Sterigmatocystin (STC) is structurally close to the mycotoxin aflatoxin B1 as it shares its biosynthetic pathway with aflatoxins. The purpose of the present study was to investigate the short-term (24 h) effects of STC contaminated diet at different doses (1 mg, 2 mg and 4 mg STC kg-1 feed) in one year old common carp juveniles. Liver samples were taken in 8-h intervals. The markers of the lipid peroxidation showed moderate changes after the application of sterigmatocystin-contaminated diet, significant elevations were only observed in the lowest applied dose group of sterigmatocystin after 16 h of exposure. Reduced glutathione content showed higher levels than control group after 16 h of exposure as effect of low dose of sterigmatocystin. Glutathione peroxidase (GPX4) activity was lower than control in the group treated with 2 mg STC kg-1 feed after 24 h of exposure. Gene expression measurements of keap1, nrf2, gpx4a, gpx4b and gss genes revealed a dual response. Down-regulation or near control values were observed 8 h after exposure which was followed by an induction 16 and 24 h after exposure. In case of gsr, gene expression values returned to...
Source: Toxicon - Category: Toxicology Authors: Tags: Toxicon Source Type: research