TaDa! Analysing cell type-specific chromatin in vivo with Targeted DamID.

TaDa! Analysing cell type-specific chromatin in vivo with Targeted DamID.
Curr Opin Neurobiol. 2019 Mar 04;56:160-166
Authors: van den Ameele J, Krautz R, Brand AH
Abstract
The emergence of neuronal diversity during development of the nervous system relies on dynamic changes in the epigenetic landscape of neural stem cells and their progeny. Targeted DamID (TaDa) is proving invaluable in identifying the genome-wide binding sites of chromatin-associated proteins in vivo, without fixation, cell isolation, or immunoprecipitation. The simplicity and efficiency of the technique have led to an ever-expanding TaDa toolbox. These tools enable profiling of gene expression and chromatin accessibility, as well as the identification of the genome-wide binding sites of chromatin complexes, transcription factors and RNAs. Here, we review these new developments, with particular emphasis on the use of TaDa in studying neuronal specification.
PMID: 30844670 [PubMed - as supplied by publisher]

Source: Current Opinion in Neurobiology - March 3, 2019 Category: Neurology Authors: van den Ameele J, Krautz R, Brand AH Tags: Curr Opin Neurobiol Source Type: research