miR-203 inhibits the invasion and EMT of gastric cancer cells by directly targeting Annexin A4.

miR-203 inhibits the invasion and EMT of gastric cancer cells by directly targeting Annexin A4. Oncol Res. 2019 Mar 05;: Authors: Li J, Zhang B, Cui J, Liang Z, Liu K Abstract Many studies have shown that down-regulated miR-203 level is in a variety of cancers including gastric cancer (GC). However, the precise molecule mechanisms of miR-203 in GC have not been well clarified. In current study, we investigated the biological functions and molecular mechanisms of miR-203 in GC cell lines. We found that miR-203 is down-regulated in GC tissues and cell lines. Moreover, the low level of miR-203 was associated with increased expression of Annexin A4 in GC tissues and cell lines. The invasion and EMT of GC cells was suppressed by overexpression of miR-203. However, down-regulation of miR-203 promoted invasion and EMT of GC cells. Bioinformatics analysis predicted that the Annexin A4 was a potential target gene of miR-203. Next, luciferase reporter assay confirmed that miR-203 could directly target Annexin A4. Consistent with the effect of miR-203, down-regulation of Annexin A4 by siRNA inhibited the invasion and EMT of GC cells. Introduction of Annexin A4 in GC cells partially blocked the effects of miR-203 mimic. Introduction of miR-203 directly targeted Annexin A4 to inhibit the invasion and EMT of GC cells. Overall, re-activation of the miR-203/Annexin A4 axis may represent a new strategy for overcoming metastasis of GC. PMID: 3...
Source: Oncology Research - Category: Cancer & Oncology Tags: Oncol Res Source Type: research