A balanced game: chicken macrophage response to ALV-J infection

In this study, we discovered an interesting phenomenon: ALV-J replication is weakened from 3  hours post-infection (hpi) to 36 hpi, which was verified using Western blotting and RT-PCR. To further investigate the interaction between ALV-J and macrophages, transcriptome analysis was performed to analyze the host genes’ function in chicken primary monocyte-derived macrophages (MDM). Compa red to the uninfected control, 624 up-regulated differentially expressed genes (DEG) and 341 down-regulated DEG at 3 hpi, and 174 up-regulated DEG and 87 down-regulated DEG at 36 hpi were identified in chicken MDM, respectively. ALV-J infection induced strong innate immune responses in chicken MDM a t 3 hpi, instead of 36 hpi, according to the analysis results of Gene Ontology and KEGG pathway. Importantly, the host factors, such as up-regulatedMIP-3 α,IL-1 β,iNOS,K60,IRG1,CH25H,NFKBIZ, lysozyme andOASL were involved in the host defense response during the course of ALV-J infection. On the contrary, up-regulatedEX-FABP,IL4I1,COX-2,NFKBIA,TNFAIP3 and the Jak STAT pathway inhibitors includingCISH,SOCS1 andSOCS3 are beneficial to ALV-J survival in chicken macrophages. We speculated that ALV-J tropism for macrophages helps to establish a latent infection in chicken MDM from 6 to 36 hpi. The present study provides a comprehensive view of the interactions between macrophages and ALV-J. It suggests the mechanisms of defense of chicken macrophages against ALV-J invasion and how ALV-J escape the ...
Source: Veterinary Research - Category: Veterinary Research Source Type: research