The SNPs in bovine MMP14 promoter influence on fat-related traits

In this study, to discover additional genetic markers associated with beef quality, we tried to identify DNA polymorphisms in bovine MMP14 gene. Consequently, two SNPs (single nucleotide polymorphisms) in the CDS (coding sequence) regions and three SNPs in the promoter region of the MMP14 gene were identified. The SNPs in the promoter region were suggested to influence on MMP14 expression, although neither quality or quantity of the MMP14 protein might undergo a change by the SNPs in the CDS regions. Statistical analysis revealed that two SNPs in the promoter region had significant effects on fat-related traits: the effect of g. -1723 bp A > C was observed on the percentage of C16:0 and that of the g. -871 bp - > T on several carcass characteristics including subcutaneous fat thickness. The percentage of C16:0 in the animals with the A/A allele in the g. -1723 bp A > C (4.22%) was higher than that of the animals with the C/C allele (3.52%). Subcutaneous fat thickness of the T/T animals of the g. -871 bp - > T (2.90 cm) was thinner than that of the −/T animals (3.14 cm), leading to higher yield estimates for the T/T homozygotes (74.10) than the heterozygotes (73.58). Our results indicate that these SNPs may be applied as genetic markers for the advancement of beef quality.

Source: Meta Gene - March 6, 2019 Category: Genetics & Stem Cells Source Type: research