Epigallocatechin-3-gallate protects the testis from damage generated by experimental cryptorchidism in rabbits.

Epigallocatechin-3-gallate protects the testis from damage generated by experimental cryptorchidism in rabbits. Histol Histopathol. 2019 Mar 04;:18096 Authors: Vigueras-Villaseñor RM, Jiménez Cabrera T, Chávez Saldaña M, Jiménez Trejo F, Cuevas Alpuche O, Rojas-Castañeda JC Abstract Cryptorchidism (CO) is a risk factor for infertility in men. It is associated with an increase in oxidative stress which alters the differentiation of the gonocytes to spermatogonia. Epigallocatechin-3-gallate (EGCG) is an antioxidant that acts as a free radical scavenger and activates the antioxidant enzymes. The aim of this work was to investigate if EGCG plays a role in the protection of the testicle from alterations generated by CO and its possible mechanism. Male rabbits 7 days old were divided into four groups and distributed as follows: 1) control (C) treated with EGCG vehicle (V) (C/V); 2) C with administration of EGCG from 65 to 120 days postpartum (dpp) (C/EGCG); 3) CO induced by administration of 17β-estradiol plus EGCG vehicle (CO/V) and 4) CO plus EGCG administration (CO/EGCG). The animals were euthanized at 120 dpp and their testes were processed to evaluate lipid peroxidation, activities of superoxide dismutase (SOD) and catalase (CAT) enzymes as well as serum testosterone (T) concentrations. In addition, the rates of apoptosis, cell proliferation and histological alterations were determined. The CO/EGCG group showed a significant re...
Source: Histology and Histopathology - Category: Cytology Tags: Histol Histopathol Source Type: research