Predominance of ST22-MRSA-IV clone and Emergence of Clones for Methicillin-resistant Staphylococcus aureus clinical isolates collected from a Tertiary Teaching Hospital over a two-year period.

This study aimed to determine the genotypic and phenotypic characteristics of clinical MRSA isolates collected from a teaching hospital from 2014 - 2015. These isolates were genotyped by multilocus sequence typing (MLST), staphylococcal cassette chromosomal mec (SCCmec) typing, virulence genes detection and pulsed-field gel electrophoresis (PFGE) and phenotyped based on their vancomycin susceptibility profiles. The most prevalent sequence type was ST22. ST3547 was identified from a blood isolate from 2015. Three SCCmec types (III in 26.26%, IV in 70.71% and V in 3.03%) were detected. Agr type I, II, III were detected among the isolates. The most prevalent virulence genes were hemolysin (100%) and intracellular adhesion (91.9%). At least one staphylococcal enterotoxins was detected in 83 (83.8%) of the isolates. All of the isolates were susceptible to vancomycin (MIC ≤ 2 µg/ml). Statistical analysis revealed significant increase in hypertension (p = 0.035), dyslipidemia and obesity (p = 0.046), and previous exposure to any quinolone (p = 0.010) cases over the two-year period. Emergence and circulation of community-associated MRSA variants were observed in our hospital. PMID: 30814457 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research