DNA polymerase {theta} accomplishes translesion synthesis opposite 1,N6-ethenodeoxyadenosine with a remarkably high fidelity in human cells [Research Communications]

Here we show that translesion synthesis (TLS) opposite 1,N6-ethenodeoxyadenosine (dA), which disrupts Watson–Crick base pairing, occurs via Pol/Pol-, Rev1-, and Pol-dependent pathways. The requirement of Pol/Pol is consistent with the ability of Pol to incorporate nucleotide opposite dA by Hoogsteen base pairing and of Pol to extend synthesis. Rev1 polymerase and Pol conduct TLS opposite dA via alternative error-prone pathways. Strikingly, in contrast to extremely error-prone TLS opposite dA by purified Pol, it performs predominantly error-free TLS in human cells. Reconfiguration of the active site opposite dA would provide Pol the proficiency for error-free TLS in human cells.

Source: Genes and Development - March 1, 2019 Category: Genetics & Stem Cells Authors: Yoon, J.-H., Johnson, R. E., Prakash, L., Prakash, S. Tags: Research Communications Source Type: research