Significance of previtamin D chromatographic resolution in the accurate determination of vitamin D3 by HPLC‒UV

This study identified four chromatographic columns (ACE C18, ACE C18 AR, Vydac 201 T P C18 and Polaris C18-Ether) with adequate selectivity that should be applied for food testing and further confirmed that both parent vitamins isomerise at the same rate under thermal conditions.
Source: Journal of Food Composition and Analysis - Category: Food Science Source Type: research