GSE111079 Characterization of murine hepatoma cells with regard to their dependence on cyclin E1

Contributors : Roland Sonntag ; Nives Giebeler ; Yulia A Nevzorova ; J örg-Martin Bangen ; Dirk Fahrenkamp ; Daniela Lambertz ; Ute Haas ; Wei Hu ; Nikolaus Gassler ; Francisco J Cubero ; Gerhard Müller-Newen ; Ali T Abdallah ; Ralf Weiskirchen ; Fabio Ticconi ; Ivan G Costa ; Mariano Barbacid ; Christian Trautwein ; Christian LiedtkeSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn C57BL/6 mice with a floxed cyclin E1 (CcnE1) gene (CcnE1f/f), hepatocellular carcinomas were induced using diethylnitrosamine (DEN). Hepatocyte-derived cells from pre-cancerous but tumor-free liver tissue (referred to as CcnE1f/fpreCL) and from large, solid HCC nodules (referred to as CcnE1f/fHCC) were isolated and immortalized in vitro. Deletion of CcnE1 in CcnE1f/fpreCL cells resulted in cell cycle arrest and death while CcnE1f/fHCC cells grew almost normal after loss of CcnE1. The underlying differences of both cell types regarding the transcriptome were analyzed in the present experiment.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research