Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): complete apicoplast genome and multiple mitochondrion-associated sequences.

Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): complete apicoplast genome and multiple mitochondrion-associated sequences. Int J Parasitol. 2019 Feb 18;: Authors: Léveillé AN, Baneth G, Barta JR Abstract Extrachromosomal genomes of the adeleorinid parasite Hepatozoon canis infecting an Israeli dog were investigated using next-generation (NGS) and standard sequencing technologies. A complete apicoplast genome and several mitochondrion-associated sequences were generated. The apicoplast genome (31,869 bp) possessed two copies of both large subunit (23S) and small subunit (16S) ribosomal RNA genes (rDNA) within an inverted repeat (IR) region, as well as 22 protein-coding sequences (CDS), 25 transfer RNA genes (tDNA) and seven open reading frames (ORF) of unknown function. Although circular-mapping, the apicoplast genome was physically linear according to NGS data. Unlike other apicoplast genomes, genes encoding ribosomal protein S19 and tDNAs for alanine, aspartic acid, histidine, threonine and valine were not identified. No complete mitochondrial genome was recovered using NGS data or directed PCR amplifications. Eight mitochondrion-associated (215 to 3,523 bp) contigs assembled from NGS data encoded complete cytochrome c oxidase subunit I CDS, complete cytochrome c oxidase subunit III CDS, two complete cytochrome B CDS, a non-coding, pseudogene for cytochrome B and multiple fragmented mitochond...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research