Application and future perspective of CRISPR/Cas9 genome editing in fruit crops.

Application and future perspective of CRISPR/Cas9 genome editing in fruit crops.
J Integr Plant Biol. 2019 Feb 21;:
Authors: Zhou J, Li D, Wang G, Wang F, Kunjal M, Joldersma D, Liu Z
Abstract
Fruit crops, including apple, orange, grape, banana, strawberry, watermelon, kiwifruit and tomato, not only provide essential nutrient for human life but also contribute to major agricultural output and economic growth of many countries and regions in the world. Recent advancement in genome editing provides an unprecedented opportunity for the genetic improvement of these agronomically important fruit crops. Here, we summarize recent reports of applying CRISPR/Cas9 to fruit crops, including efforts to reduce disease susceptibility, change plant architecture or flower morphology, improve fruit quality traits, and increase fruit yield. We discuss challenges facing fruit crops as well as new improvements and platforms that could be used to facilitate genome editing in fruit crops, including dCas9-base-editing to introduce desirable alleles and heat treatment to increase editing efficiency. In addition, we highlight what we see as potentially revolutionary development ranging from transgene-free genome editing to de novo domestication of wild relatives. Without doubt, we now see only the beginning of what will eventually be possible with the use of CRISPR/Cas9 toolset. Efforts to communicate with the public and an emphasis on the manipulation of co...

Source: Journal of Integrative Plant Biology - February 21, 2019 Category: Biology Authors: Zhou J, Li D, Wang G, Wang F, Kunjal M, Joldersma D, Liu Z Tags: J Integr Plant Biol Source Type: research