High-intensity interval training increase GATA4, CITED4 and c-Kit and decreases C/EBPβ in rats after myocardial infarction

Publication date: Available online 22 February 2019Source: Life SciencesAuthor(s): Nasim Naderi, Mohammad Hemmatinafar, Abbas Ali Gaeini, Aida Bahramian, Alireza Ghardashi Afousi, Mohammad Reza Kordi, Amir Darbandi-Azar, Fariba Karimzade, Hamid Mohebbi, Mahmood BaratiAbstractAimMyocardial infarction (MI), an important cause of morbidity and mortality, can be followed by left ventricular dysfunction and cardiomyocyte loss. Cardiac repair mechanisms may subsequently improve left ventricular function. Exercise training has been suggested to have cardioprotective effects against MI damage, but detailed knowledge is lacking on the effects of different types and intensities of exercise training on molecular targets of cardiomyocyte regeneration.Main methodsMI was induced in male Wistar rats by ligating the left anterior descending coronary artery. After MI induction, the rats were randomly assigned to one of five groups: sham operated, and experimental MI followed by no exercise, or low, moderate or high intensity exercise Cardiac function and infarct size were assessed by echocardiography and Evans blue/TTC staining, respectively. The expression of mRNA markers and proteins associated with myocardial regeneration was measured with RT-PCR and western blotting.Key findingsExercise training at different intensities improved cardiac function and levels of stem cell and cardiomyocyte markers, and reduced infarct size. mRNA levels of GATA4, Nkx2.5 and c-Kit and protein expression of Nkx...
Source: Life Sciences - Category: Biology Source Type: research