Expression of Notch-1 and nuclear factor- κB signal pathway in myocardial cells of coronary heart disease rats.

Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats. Exp Ther Med. 2019 Mar;17(3):1587-1592 Authors: Zheng J, Zhang S, Wang T Abstract Expression changes of Notch-1 and nuclear factor-κB (NF-κB) in cardiomyocytes from coronary heart disease (CHD) rats were investigated. The CHD model established by high-fat diet in 48 clean SD rats was set as the observation group, and another 48 healthy rats routinely fed were the control group. Rats were sacrificed on the 1st, 3rd, 7th, and 14th days after successful modeling. The rat myocardial cells were harvested to examine the changes of Notch-1 and NF-κB using immunoblot (western blot analysis) and TUNEL assay. Cardiomyocyte apoptotic rate, by Pearson's correlation test was used to analyze the correlation between Notch-1 and NF-κB. With the prolongation of the course of CHD in rats, the expression levels of Notch-1 and NF-κB proteins gradually increased, and the expression in the observation group was significantly higher than that in the control group (P<0.01). On the seventh day, the expression levels of Notch-1 and NF-κB protein in the rats in the observation group showed significant difference from those on day 1 and day 3 (P<0.05), and they were significantly different on the 7th and 14th days (P<0.05). There was no correlation between the expression level of the two proteins with the age and sex of the rats. Pearso...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research