Viruses, Vol. 11, Pages 188: Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System

Viruses, Vol. 11, Pages 188: Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System
Viruses doi: 10.3390/v11020188
Authors:
Angela Victoria Holguín
Pablo Cárdenas
Catalina Prada-Peñaranda
Laura Rabelo Leite
Camila Buitrago
Viviana Clavijo
Guilherme Oliveira
Pimlapas Leekitcharoenphon
Frank Møller Aarestrup
Martha J. Vives

Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage. Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution. This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dyn...

Source: Viruses - February 22, 2019 Category: Virology Authors: Angela Victoria Holgu ín Pablo C árdenas Catalina Prada-Pe ñaranda Laura Rabelo Leite Camila Buitrago Viviana Clavijo Guilherme Oliveira Pimlapas Leekitcharoenphon Frank M øller Aarestrup Martha J. Vives Tags: Article Source Type: research