The structure of CgnJ, a domain of unknown function protein from the crocagin gene cluster

Natural products often contain interesting new chemical entities that are introduced into the structure of a compound by the enzymatic machinery of the producing organism. The recently described crocagins are novel polycyclic peptides which belong to the class of ribosomally synthesized and post-translationally modified peptide natural products. They have been shown to bind to the conserved prokaryotic carbon-storage regulator A in vitro. In efforts to understand crocagin biosynthesis, the putative biosynthetic genes were expressed and purified. Here, the first crystal structure of a protein from the crocagin-biosynthetic gene cluster, CgnJ, a domain of unknown function protein, is reported. Possible functions of this protein were explored by structural and sequence homology analyses. Even though the sequence homology to proteins in the Protein Data Bank is low, the protein shows significant structural homology to a protein with known function within the competency system of Bacillus subtilis, ComJ, leading to the hypothesis of a similar role of the protein within the producing organism.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: crocagin RiPPs CgnJ domains of unknown function research communications Source Type: research

Related Links:

Conclusion: The Readmission rate was 13.7%, with an associated mortality of 27.8%. Early readmission was an independent predictor of mortality (RR 1.95) in relation to late readmission. Larger studies are needed to better identify this group of patients with an aim to adopt preventive measures.
Source: Quality Management in Healthcare - Category: Health Management Tags: Original Research Source Type: research
The State of Washington received a State Innovation Models (SIM) $65 million award from the federal Centers for Medicare &Medicaid Services to improve population health and quality of care and reduce the growth of health care costs in the entire state, which has over 7 million residents. SIM is a “complex intervention” that implements several interacting components in a complex, decentralized health system to achieve goals, which poses challenges for evaluation. Our purpose is to present the state-level evaluation methods for Washington's SIM, a 3-year intervention (2016-2018). We apply the RE-AIM (reach, e...
Source: Quality Management in Healthcare - Category: Health Management Tags: Evaluation Design Source Type: research
Conclusion: This project found that there were not significant differences in ED LOS for injured patients presenting initially to FSEDs or other non-FSED facilities, suggesting that timeliness of care was similar across location types.
Source: Quality Management in Healthcare - Category: Health Management Tags: Quality Management Applications Source Type: research
Conclusion: The use of a best practice quality improvement process resulted in improvements in workflow related to SBIRT, greater communication about SBIRT, and identification of barriers that blocked successful receipt of SBIRT.
Source: Quality Management in Healthcare - Category: Health Management Tags: Quality Management Applications Source Type: research
Conclusions: Multifactor designs are not widely used in medicine despite their potential to make improvement initiatives and health services research more efficient and effective. Quality managers, health system leaders, and directors of research institutes could increase productivity and gain important insights by promoting a broader use of factorial designs to study multiple interventions simultaneously and to learn from interactions.
Source: Quality Management in Healthcare - Category: Health Management Tags: Perspectives Source Type: research
No abstract available
Source: Quality Management in Healthcare - Category: Health Management Tags: Intermountain Advances Source Type: research
No abstract available
Source: Quality Management in Healthcare - Category: Health Management Tags: Insights From the Armstrong Institute Source Type: research
No abstract available
Source: Quality Management in Healthcare - Category: Health Management Tags: Information for Authors Source Type: research
No abstract available
Source: Quality Management in Healthcare - Category: Health Management Tags: Call for Papers Source Type: research
No abstract available
Source: Quality Management in Healthcare - Category: Health Management Tags: Call for Reviewers Source Type: research
More News: Biochemistry | Chemistry | Genetics