Molecules, Vol. 24, Pages 785: Flavopereirine —An Alkaloid Derived from Geissospermum vellosii—Presents Leishmanicidal Activity In Vitro

Molecules, Vol. 24, Pages 785: Flavopereirine—An Alkaloid Derived from Geissospermum vellosii—Presents Leishmanicidal Activity In Vitro Molecules doi: 10.3390/molecules24040785 Authors: da Silva e Silva Cordovil Brigido Oliveira de Albuquerque Miranda Carvalho Ferreira Reis Vinhal Faria Coelho-Ferreira Silveira da Silva Carneiro Percário do Rosário Marinho Dolabela Chemotherapy is limited in the treatment of leishmaniasis due to the toxic effects of drugs, low efficacy of alternative treatments, and resistance of the parasite. This work assesses the in vitro activity of flavopereirine on promastigote cultures of Leishmania amazonensis. In addition, an in silico evaluation of the physicochemical characteristics of this alkaloid is performed. The extract and fractions were characterized by thin-layer chromatography and HPLC-DAD, yielding an alkaloid identified by NMR. The antileishmanial activity and cytotoxicity were assayed by cell viability test (MTT). The theoretical molecular properties were calculated on the Molinspiration website. The fractionation made it possible to isolate a beta-carboline alkaloid (flavopereirine) in the alkaloid fraction. Moreover, it led to obtaining a fraction with greater antileishmanial activity, since flavopereirine is very active. Regarding the exposure time, a greater inhibitory effect of flavopereirine was observed at 24 h and 72 h (IC50 of 0.23 and 0.15 μg/mL, respectively). The ext...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research