Orthotopic hepatocellular carcinoma: molecular imaging-monitored intratumoral hyperthermia-enhanced direct oncolytic virotherapy.

This study included in vitro experiments using luciferase-labeled rat HCC cells and in vivo validation experiments on rat models with orthotopic HCCs. Both cells and HCCs in four groups (n = 6/group) were treated by: (1) combination therapy of oncolytic virotherapy (T-VEC) plus RFH at 42 °C for 30 min; (2) oncolytic virotherapy alone; (3) RFH alone; and (4) saline. For in vitro confirmation, confocal microscopy and bioluminescence optical imaging were used to evaluate the cell viabilities. For in vivo validation, oncolytic viruses were directly infused into rat HCCs through a multi-functional perfusion-thermal RF electrode, followed by RFH. Ultrasound and optical imaging were used to follow up size and bioluminescence signal changes of tumors overtime, which were correlated with subsequent laboratory examinations. RESULTS: For in vitro experiments, confocal microscopy showed the lowest number of viable cells, as well as a significant decrease of bioluminescence signal intensity of cells with combination therapy group, compared to other three groups (p 
Source: International Journal of Hyperthermia - Category: Internal Medicine Tags: Int J Hyperthermia Source Type: research

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Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 23 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Shahad W. Kattan, Mohamed S. Nafie, Gamal A. Elmgeed, Walla Alelwani, Muhammad Badar, Mohamed A. TantawyAbstractWorldwide, cancer still an area with high unmet medical need. Lead optimization efforts toward structure-based drug design were employed to discover newly synthesized hetero-steroid derivatives with promising anticancer effects against hepatocellular carcinoma (HCC). The aim of our study is to evaluate the anti-proliferative activity, a dual PI3K/mTOR inhibitor, and mechanism of action of ...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research
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Source: Journal of Vascular and Interventional Radiology - Category: Radiology Source Type: research
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Source: Journal of Vascular and Interventional Radiology - Category: Radiology Source Type: research
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Source: Revista Espanola de Enfermedades Digestivas - Category: Gastroenterology Tags: Rev Esp Enferm Dig Source Type: research
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Source: Life Sciences - Category: Biology Source Type: research
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Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research
Authors: Ifeorah IM, Bakarey AS, Akubo AO, Onyemelukwe FN Abstract Hepatitis C virus (HCV) is associated with liver complicated diseases resulting in end-stage hepatocellular carcinoma. Although vertical transmission from mother to child serves as one of the routes of HCV acquisition in children, yet HCV infection in pregnant women and children is still underappreciated in sub-Saharan Africa. Therefore, this study investigated the burden of HCV, associated risk factors, and viremia among antenatal and postnatal clinic attendees in the rural and urban communities of Kogi State, Nigeria. Atotal of 176 blood samples w...
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research
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Source: Pharmaceutical Biology - Category: Drugs & Pharmacology Tags: Pharm Biol Source Type: research
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