Development and Validation of an HPLC-UV Method for Simultaneous Determination of Sildenafil and Tramadol in biological fluids: Application to Drug-Drug Interaction Study

In this study, a reversed-phase chromatography was performed at room temperature on a C18 column with a mobile phase composed of 10 mM Na2HPO4 solution (pH 7.5): acetonitrile (45:55, v/v) at a flow rate of 0.8 mL min-1 using caffeine (CAF) as an internal standard. The detector was set at 220 nm. The total analysis time was 6 min. Calibration graphs were linear in the concentration ranges of 0.1–10 and 0.05–10 µg mL-1 with a detection limit of 0.05 and 0.02 µg mL-1 for TMD and SDF, respectively. The method was validated in terms of accuracy, precision, limit of detection and quantitation, recovery, and stability as per US FDA bioanalytical guidelines. In addition, the metabolites N-desmethylsildenafil (UK-103,320) and O-desmethyltramadol were quantified in rabbit plasma after 2 h of oral administration using LC–MS/MS. The simultaneous administration of TMD with SDF has affected peak plasma concentration (Cmax), Tmax, area under the concentration-time curve (AUC), and the elimination rate constant (Kel) of SDF. The present study is the first to give valuable insights into the drug-drug interaction and the pharmacokinetic implications associated with the co-administration of SDF and TMD.Graphical Abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Today erectile dysfunction (ED) is a commonly known medical condition with various effective therapies, but this was not always the case. When I was in medical school (1963-1968) this condition fell under a rubric called impotence. Our understanding of impotence and our ability to effectively manage it was almost nonexistent. The urology text used when I was a student had the following to say about impotence: “Various degrees of impotence in men are common, but it is rare to find definite organic cause for the complaints, which include inability to gain an erection, weak erections, premature ejaculation, loss of libi...
Source: Urology - Category: Urology & Nephrology Authors: Tags: Commentary Source Type: research
Today erectile dysfunction (ED) is a commonly known medical condition with various effective therapies, but this was not always the case. When I was in medical school (1963-1968) this condition fell under a rubric called impotence. Our understanding of impotence and our ability to effectively manage it was almost nonexistent. The urology text used when I was a student had the following to say about impotence: “Various degrees of impotence in men are common, but it is rare to find definite organic cause for the complaints, which include inability to gain an erection, weak erections, premature ejaculation, loss of libi...
Source: Urology - Category: Urology & Nephrology Authors: Source Type: research
Publication date: November 2019Source: Epilepsy &Behavior, Volume 100, Part AAuthor(s): Chaturbhuj Rathore, Oliver J. Henning, Gerhard Luef, Kurupath RadhakrishnanAbstractSexual dysfunction is a common comorbidity in people with epilepsy (PWE) that adversely affects their quality of life. Nearly one-half of men and women with epilepsy have sexual dysfunction, but in the majority, this often goes unnoticed. The wide variation in the reported prevalence of sexual dysfunction in PWE is due to the significant heterogeneity among the studies with regard to patient population, type and severity of epilepsy, number and type o...
Source: Epilepsy and Behavior - Category: Neurology Source Type: research
Abstract Sexual dysfunction is a common comorbidity in people with epilepsy (PWE) that adversely affects their quality of life. Nearly one-half of men and women with epilepsy have sexual dysfunction, but in the majority, this often goes unnoticed. The wide variation in the reported prevalence of sexual dysfunction in PWE is due to the significant heterogeneity among the studies with regard to patient population, type and severity of epilepsy, number and type of antiseizure drugs (ASDs) used, and the tools used for assessing sexual dysfunction. Generally, patients with uncontrolled epilepsy, longer duration of epil...
Source: Epilepsy and Behaviour - Category: Neurology Authors: Tags: Epilepsy Behav Source Type: research
Doctors know that erectile dysfunction is common, but calculating its exact prevalence is tough. A recent review has attempted to draw a clearer picture.
Source: Health News from Medical News Today - Category: Consumer Health News Tags: Erectile Dysfunction / Premature Ejaculation Source Type: news
In the first study of its kind, experts from James Cook University in Australia analysed data from 650 men who used pelvic floor exercises to combat their ED and premature ejaculation.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Authors: Blum C, Chesnel C, Hentzen C, Turmel N, Lacroix P, Manceau P, Amarenco G Abstract INTRODUCTION: To analyze in a descriptive way the ejaculation disorders during MS and to study the correlations between dysejaculations, other sexual, urinary and anorectal disorders. MATERIAL: Prospective, monocentric, non-interventional study. Ejaculation disorders were assessed by Premature Ejaculation Profile (PEP) scores, intravaginal latency, and the Male Sexual Health Questionnaire - Ejaculation (MSHQ-Ej). The sexual disorders were evaluated by the International Index of Erectile Function (IIEF15) and the Sclerosis...
Source: Progres en Urologie - Category: Urology & Nephrology Tags: Prog Urol Source Type: research
Erectile dysfunction (ED) and premature ejaculation (PE) often have underlying musculoskeletal abnormalities. Despite this, traditional management has focused on pharmaceutical prescription.
Source: Physiotherapy - Category: Physiotherapy Authors: Tags: Systematic review Source Type: research
A new, large-scale genomic analysis suggests that having a genetic predisposition to type 2 diabetes may cause erectile dysfunction.
Source: Health News from Medical News Today - Category: Consumer Health News Tags: Erectile Dysfunction / Premature Ejaculation Source Type: news
ConclusionsErectile dysfunction ‐PE patients present several similarities with those consulting only for ED, whereas their characteristics are different from PE‐only men. In agreement with the guidelines, our results confirm that ED‐PE men might be considered (and managed) primarily as patients with ED.
Source: Andrology - Category: Urology & Nephrology Authors: Tags: Original Article Source Type: research
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