GSE126718 Mycobacterium tuberculosis transcriptional response to small molecule

Contributors : Wang Xin ; Hussain SeemaSeries Type : Expression profiling by high throughput sequencingOrganism : Mycobacterium tuberculosisWe applied RNAseq (Nextseq) technology to study mechanism of action of nitro-containing heterocycle antitubercular JSF-2019, with des-nitro JSF-2026 as control. Briefly, mid-log phase (OD = 0.3) M. tuberculosis culture was treated by 10x MIC of each compound in biological quadruplicates followed by mRNA extraction and RNAseq analysis. We find that JSF-2019 and pretomanid as intracellular NO • donors exhibited distinct transcriptional patterns comparing to extracellular iNOS inducer such as DETA/NO, therefore it suggests distinguished mechanisms of action between intracellular vs. extracellular NO• donors. We also observed that JSF-2019 upregulated a subset of FAS-II genes similar t o isoniazid, indicating JSF-2019 inhibits intracellular mycolic acid biosynthesis.

Source: GEO: Gene Expression Omnibus - February 20, 2019 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mycobacterium tuberculosis Source Type: research