Individual and combined cytotoxicity of major trichothecenes type B, deoxynivalenol, nivalenol, and fusarenon-X on Jurkat human T cells.

Individual and combined cytotoxicity of major trichothecenes type B, deoxynivalenol, nivalenol, and fusarenon-X on Jurkat human T cells.
Toxicon. 2019 Feb 15;:
Authors: Aupanun S, Phuektes P, Poapolathep S, Alassane-Kpembi I, Oswald IP, Poapolathep A
Abstract
Food and feed commodities are often contaminated by more than one mycotoxin. Among the several combinations that frequently occur, fusariotoxins are often mentioned. The multi-mycotoxins contamination is a serious threat for health. In the present study we investigated the toxic interactions between deoxynivalenol (DON), nivalenol (NIV), and fusarenon-X (FX) in Jurkat T cells by using the MTT assay. The tested mycotoxins alone or in combination had a dose dependent effect on proliferating lymphocytes. According to IC50, it could be classified in decreasing order of toxicity: FX > NIV > DON > DON+FX > NIV+FX > DON+NIV > DON+NIV+FX. The type of mycotoxin interactions was assessed by calculating the combination index (CI) and the dose reduction index (DRI). Our data indicate that an antagonistic effect was strongly observed in binary combination between DON and NIV at higher concentrations and ternary mixtures. Meanwhile, the DON and FX mixtures generated moderate antagonism, while NIV and FX provoked different interactions. The effects of tested mycotoxins on apoptosis were also determined using a FACScan flow cytometer. At IC75, the percentages of apoptotic cell...

Source: Toxicon - February 15, 2019 Category: Toxicology Authors: Aupanun S, Phuektes P, Poapolathep S, Alassane-Kpembi I, Oswald IP, Poapolathep A Tags: Toxicon Source Type: research