Generation of a human induced pluripotent stem cell line (MUi010-A) from skin fibroblast of patient carrying a c.2104C>T mutation in MYH9 gene

Publication date: Available online 18 February 2019Source: Stem Cell ResearchAuthor(s): Amornrat Tangprasittipap, Bunyada Jittorntrum, Praguywan Kadegasem, Werasak Sasanakul, Nongnuch Sirachainan, Narisorn Kitiyanant, Suradej HongengAbstractMutations in MYH9 gene is one of the major causes of inherited thrombocytopenia resulted from nonfunctional myosin-9 protein. We have generated a human induced pluripotent stem cell line MUi010-A from skin fibroblasts of a patient who had a point mutation c.2104C>T (p.R702C) in the exon 16 of MYH9 gene using a non-integrative reprogramming method. The MUi010-A exhibited embryonic stem cell-like characteristics with consistent pluripotent markers expression, was capable of all three embryonic germ layers differentiation, and had a normal karyotype.
Source: Stem Cell Research - Category: Stem Cells Source Type: research