Synergistic effect of phototherapy and chemotherapy on bladder cancer cells

Publication date: Available online 18 February 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Mehdi Shakibaie, Maryam Vaezjalali, Hashem Rafii-Tabar, Pezhman SasanpourAbstractDrug resistance as an important barrier in cancer treatment, has a close relation with alteration of cancer metabolism. Therefore, in this study the synergistic effect of phototherapy and chemotherapy were investigated on bladder cancer cells viability. The cytotoxicity effect of blue light irradiation was measured by MTT assay. Glucose consumption, lactate and ammonium formation were analyzed in blue LED-irradiated cancer cells culture. Also, the expression of some genes involved in apoptosis and epithelial–mesenchymal transition was assessed using real-time PCR in comparison with control group. The analysis of the results indicated that blue light irradiation inhibited the cell viability in a dose-dependent manner. Blue light irradiation decreased the cell viability by 7% and 19% (p < .05) in 5637 cells at doses of 8.7 J/cm2 and 17.5J/cm2 in comparison with control group respectively. Glucose consumption, lactate and ammonium formation diminished in blue LED-irradiated 5637 cells in both doses. The real time PCR results indicated that the expression of Bax increased in blue light-irradiated cells. In addition, the cell cycle analysis showed that blue light irradiation arrested the bladder cancer in G1 phase. Also, the effect of combination therapy on cancer cells ...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research