Diversity of Nontuberculous Mycobacteria In Kuwait: Rapid Identification And Differentiation Of Mycobacterium species by multiplex PCR, INNO-LiPA Mycobacteria v2 assay and PCR-sequencing of rDNA

Conclusions: mPCR rapidly differentiated MTB from NTM. LiPA identified 44 of 52 NTM isolates at species/species complex-level and two mixed cultures. PCR-sequencing yielded more specific identification at species/subspecies-level. Rapid differentiation as MTB or NTM by mPCR, followed by species-specific NTM identification by LiPA/PCR-sequencing is suitable for proper management of mycobacterial infections in Kuwait.
Source: Medical Principles and Practice - Category: Internal Medicine Source Type: research