Anti ‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis.

Anti‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis. Mol Med Rep. 2019 Jan 22;: Authors: Choo GS, Lim DP, Kim SM, Yoo ES, Kim SH, Kim CH, Woo JS, Kim HJ, Jung JY Abstract Dendropanax morbifera (D. morbifera), known as Dendro, means 'omnipotent drug' (Panax), and has been called the panacea tree. Various studies on D. morbifera are currently ongoing, aiming to determine its medicinal uses. The present study investigated the anti‑inflammatory effects and underlying mechanism of a natural extract of D. morbifera leaves (DPL) in lipopolysaccharide (LPS)‑stimulated RAW264.7 macrophages. In the present study, the following assays and models were used: MTT assay, nitric oxide (NO) assay, western blotting, ELISA and mouse models of atopic dermatitis. DPL extract markedly reduced the production of NO, inducible NO synthase and interleukin‑6, as well as the nuclear translocation of nuclear factor‑κB (NF‑κB). Additionally, the LPS‑induced activation of extracellular signal‑regulated kinase 1/2 (ERK1/2), P38 and c‑Jun N‑terminal kinase (JNK) was suppressed by DPL extract. Taken together, these results indicate that NF‑κB, ERK1/2, P38 and JNK may be potential molecular targets of DPL extract in the LPS‑induced inflammatory response. Subsequently, the present study investigated the effects of DPL extract in a 2,4‑din...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research