Molecular investigation of tick-borne pathogens in ixodid ticks infesting domestic animals (cattle and sheep) and small rodents (black rats) of Corsica, France

This study aimed to investigate the presence and perform molecular characterization of Anaplasmataceae, Rickettsia spp., and Borrelia burgdorferi sensu lato (sl) in tick species collected in Corsica. Ticks from cattle (Bos taurus), sheep (Ovis aries), and rodents (Rattus rattus) were collected from May to September 2016. DNA was purified from ticks, submitted to quantitative real-time polymerase chain reaction (qPCR) and sequenced for phylogenetic analysis. In total, 660 ticks were collected from 111 animals during the study. The most abundant collected tick species from cattle was Rhipicephalus bursa (n = 495; 84.5%), followed by Hyalomma marginatum (n = 91; 15.5%). Rhipicephalus bursa and Ixodes ricinus were the only tick species collected from sheep and rodents, respectively. Overall, Rickettsia was the most common pathogen group (n = 48; 24%) detected in ticks. Sequence analysis of partial gltA and ompA genes revealed the presence of Ri. aeschlimannii and Candidatus Ri. barbariae. Anaplasmataceae DNA was detected in eight (6%) of the 127 cattle pools and in one (2%) of the 61 R. bursa specimens collected from sheep. Sequence analysis of the rpoB gene revealed the presence of one Anaplasma species, A. marginale.Borrelia burgdorferi sl DNA was detected in one pool of H. marginatum collected from cattle and in two (15%) of the 13 I. ricinus pools collected from nine black rats. To our knowledge, this is the first report of the occurr...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research

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(Virginia Tech) Renowned tick immunobiologist Utpal Pal wants to adapt the rabies vaccination platform to produce antibodies that can protect against Borrelia burgdorferi, the bacteria responsible for Lyme disease. The intention is to apply this work to other tick-borne diseases in the future.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Infection with tick borne Borrelia Burgdorferi (Lyme disease) can without treatment rarely develop into a chronic phase. Secondary Normal Pressure Hydrocephalus (sNPH) based on chronic infection with Borrelia ...
Source: BMC Neurology - Category: Neurology Authors: Tags: Case report Source Type: research
Abstract Companion animals can become infected with tick-borne diseases (TBDs) and can become hosts of transmission to humans, thereby damaging human health. To clarify whether companion animals are infested by ticks harboring TBD pathogens in humans, we detected TBD pathogens in ticks collected from dogs and cats brought to animal hospitals in Yamagata Prefecture, Japan. Investigation of 164 adult ticks collected from 88 dogs and 41 cats during March-July 2018 revealed the predominant tick species as Ixodes ovatus (n = 95, 57.9%), followed by Ixodes nipponensis (37, 22.6%) and Haemaphysalis flava (10, 6.1%). The ...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research
Date: Friday, 08 21, 2020; Speaker: Travis Bourret, Ph.D., Assistant Professor, Department of Medical Microbiology&Immunology, Creighton University School of Medicine; Building: Online - Webex, twitter, Facebook Live; URL:
Source: NIH Calendar of Events - Category: American Health Source Type: events
Ticks and mosquitoes don’t care about COVID-19 safety protocols. They don’t care that people are trying to squeeze out the last moments of this restrictive summer by getting outdoors, hiking, or just sitting on their decks at night and feeling something that’s close to normal. COVID-19 has commanded our attention and caused people to adapt their behaviors to prevent one major health concern, but it doesn’t mean others have been eliminated. “Masks and social distancing will do nothing to protect you from what ticks and mosquitoes potentially carry,” says Dr. Todd Ellerin, director of infe...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Autoimmune diseases Prevention Safety Source Type: blogs
Publication date: Available online 24 July 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Annetta Zintl, Taher Zaid, Fiona McKiernan, Amalia Naranjo-Lucena, Jeremy Gray, Sorcha Brosnan, John Browne, Jack O’Connor, John Mee, Barbara Good, Emma L. Gillingham, Alexander G.C. Vaux, Jolyon Medlock
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Lyme disease (LD) is an increasing public health problem. Current laboratory testing is insensitive in early infection, the stage at which appropriate treatment is most effective in preventing disease sequelae. The Lyme Disease Biobank (LDB) collects samples from individuals with symptoms consistent with early LD presenting with or without erythema migrans (EM) or an annular, expanding skin lesion and uninfected individuals from areas of endemicity. Samples were collected from 550 participants (298 cases and 252 controls) according to institutional review board-approved protocols and shipped to a centralized biorepository....
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research
In this study, multilocus sequence typing (MLST) was employed to investigate the local population structure of B. garinii in Ixodes ricinus ticks. The study took place in a natural wetland in Slovakia, temporally encompassing spring and autumn bird migration periods as well as the breeding period of resident birds. In total, we examined 369 and 255 ticks collected from 78 birds and local vegetation, respectively. B. burgdorferi s.l. was detected in 43.4% (160/369) of ticks recovered from birds and in 26.3% (67/255) of questing ticks, respectively. Considering the ticks from bird hosts, the highest prevalence was found for ...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Committee Opinion No. 399: Management of Tick Bites and Lyme Disease During Pregnancy: (En français : Prise en charge des morsures de tiques et de la maladie de Lyme pendant la grossesse). J Obstet Gynaecol Can. 2020 May;42(5):644-653 Authors: Smith GN, Moore KM, Hatchette TF, Nicholson J, Bowie W, Langley JM Abstract OBJECTIVE: Lyme disease is an emerging infection in Canada caused by the bacterium belonging to the Borrelia burgdorferi sensu lato species complex, which is transmitted via the bite of an infected blacklegged tick. Populations of blacklegged ticks continue to expand and are now es...
Source: Journal of Obstetrics and Gynaecology Canada : JOGC - Category: OBGYN Tags: J Obstet Gynaecol Can Source Type: research
Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferi sensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi. The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in patients with early Lyme infection. Asymptomatic subjects from endemic and nonendemic origins demonstrated a seroreactivity rate of approximately 4% that was similar to other commercial assays evaluated in this study. Coupled to this result was the observation that the Lyme Total assay retained a high first-t...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research
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