Syringa vulgaris bark as a source of compounds affecting the release of inflammatory mediators from human neutrophils and monocytes/macrophages

Publication date: Available online 13 February 2019Source: Phytochemistry LettersAuthor(s): Agnieszka Filipek, Joanna Wyszomierska, Barbara Michalak, Anna K. KissAbstractIn traditional European medicine, common lilac (Syringa vulgaris L., Oleaceae) bark has been used in the form of an infusion, decoction or alcoholic extract as an antipyretic and a cold and cough treatment. Our primary study showed that alcoholic extract of the bark was able to decrease the release of interleukin- 8 in an in vitro model The aim of the study was to investigate the phytochemical composition of common lilac bark extract by the HPLC-DAD-MS/MS method, isolate the dominant compounds and investigate their effects on the proinflammatory functions of human neutrophils and monocytes/macrophages. Syringin (sinapyl alcohol-O-glucoside), olivil-4′-O-glucoside, sinapyl aldehyde-O-glucoside and forsythoside B were isolated using RP-18, Sephadex LH-20 column chromatography and preparative chromatography. The structures were confirmed by the UV, MS and 1H and 13C NMR spectra. The effects on cytokine (TNF-α, IL-1β, IL-6) and chemokine (IL-8 and MCP-1) production by neutrophils and monocytes/macrophages were measured using an enzyme-linked immunosorbent assay (ELISA). Moderate anti-inflammatory activity was observed in the neutrophil model for all compounds compared with oleuropein, a common compound in the Oleaceae family with well-documented anti-inflammatory effects. However, syringin at concentration of...

Source: Phytochemistry Letters - February 13, 2019 Category: Chemistry Source Type: research