Fisetin loaded binary ethosomes for management of skin cancer by dermal application on UV exposed mice

Publication date: Available online 10 February 2019Source: International Journal of PharmaceuticsAuthor(s): Thasleem Moolakkadath, Mohd. Aqil, Abdul Ahad, Syed Sarim Imam, Arshiya Praveen, Yasmin Sultana, Mohd Mujeeb, Zeenat IqbalAbstractFisetin loaded binary ethosomes were prepared and optimized using Box-Behnken design for dermal application to alleviate skin cancer. The prepared formulations were evaluated for vesicle size, entrapment efficiency and flux of fisetin. Additionally, the optimized formulation was further evaluated by transmission electron microscopy, confocal laser microscopy, vesicles-skin interaction, dermatokinetic study and DPPH (2, 2-diphenyl-1-picryl-hydrazyl) assay. The in vivo study was carried out for the evaluation of tumor incidence, pro-inflammatory cytokines such as TNF-α and IL-1α, lipid peroxidation values, glutathione content and catalase activity in mice. The optimized binary ethosomes formulation presented sealed unilamellar shaped vesicles, with vesicles size, entrapment efficiency and flux of 99.89 ± 3.24 nm, 89.23 ± 2.13% and 1.01 ± 0.03 µg/cm2/h respectively. The confocal images of rat skin clearly illustrated the deeper penetration of rhodamine B loaded binary ethosomes formulation. Further, the binary ethosomes gel treated rat skin showed substantial increase in CSkin max and AUC0-8 in comparison to rat skin treated with conventional gel. In vivo study revealed that the mice pre-treated with fiseti...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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