Sequence analysis and genomic organization of a novel chuvirus, T àiyuán leafhopper virus

AbstractA novel unsegmented chuvirus was found in a leafhopper (Psammotettix alienus) and tentatively named “Tàiyuán leafhopper virus” (TYLeV). The full genome of TYLeV is 14,670 nucleotides in length and includes four ORFs encoding glycoprotein, nucleocapsid protein, RNA-dependent RNA polymerase and an uncharacterized protein, respectively. These genomic features are similar to those of the chuviru s Scaldis River bee virus, but the virus is highly divergent from known chuviruses and other members in the orderMononegavirales, with amino acid sequence identities of only 13.6 –33% for the RNA-dependent RNA polymerase (RdRp). On the basis of genomic features, phylogenetic analysis and comparisons of RdRp sequences, we suggest that this virus is a member of a new species of chuvirus in the proposed genusMivirus.

Source: Archives of Virology - February 1, 2019 Category: Virology Source Type: research