Biotechnological applications of protein splicing.

Biotechnological applications of protein splicing.
Curr Protein Pept Sci. 2019 Feb 07;:
Authors: Sarmiento C, Camarero JA
Abstract
Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturally occurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.
PMID: 30734675 [PubMed - as supplied by publisher]

Source: Current Protein and Peptide Science - February 7, 2019 Category: Biochemistry Authors: Sarmiento C, Camarero JA Tags: Curr Protein Pept Sci Source Type: research