Advances in Microfluidic Biosensors Based on Luminescent Bacteria

Publication date: February 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 2Author(s): Xiao-Wei JIN, Zhe-Yu LI, Pian-Pian XU, Xiao-Yan ZHANG, Nan-Qi REN, Vitaliy V. Kurilenko, Kai SUNAbstractLuminescent bacteria can emit visible light at 450–490 nm, and its luminous intensity decreases with the increase of the concentration of toxic substances in test solution. The method using luminescent bacteria is widely used in acute toxicity analysis of water quality because it is a simple, rapid and low-cost way. In recent years, biosensors based on luminescent bacteria have attracted more and more attention, and the reports of bioluminescence biosensors based on microfluidic systems are increasing day by day. Based on the characteristics and mechanism of luminescent bacteria, this paper introduces their applications in the environmental monitoring, and summarizes several new bioluminescence sensors.Graphical AbstractThe novel microfluidic devices for detecting toxic substances in drinking water and waste water have been developed by combining microfluidic techniques such as microchamber, micromixer and in-situ detection with the characteristics of simple, rapid and high stability of luminescent bacterial toxicity analysis.
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research