Metabolic shift of Staphylococcus aureus under sublethal dose of methicillin in the presence of glucose

This study demonstrated that a combination of glucose and a sublethal dose of antibiotic can reduce the survivability of S. aureus in a glucose concentration-dependent manner. Mass spectrometry-based targeted metabolic profiling detected massive metabolic profile shift of both methicillin-susceptible and resistant S. aureus after methicillin and glucose co-treatment. The dramatic alteration of metabolites from these metabolic pathways can be detected when 10 mg/L or higher concentration of glucose were added to methicillin treated culture. Our data also indicated that multiple biochemical metabolic pathways, including pyrimidine metabolism and valine, leucine, and isoleucine degradation showed a significant difference (p 
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: November 2019Source: Saudi Journal of Biological Sciences, Volume 26, Issue 7Author(s): Asmaa FadenAbstractWe assessed the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) strains on surfaces of hospital dental clinics. Specimens were obtained from 5 clinically symptoms-free patients of five different specialties clinics (Implantology, Pediatric Dentistry, Prosthetics, Restorative Dentistry, and Oral Medicine) of the Dental Clinic Hospital of King Saud University before and after each patient. A Q-tip swabs were used from 10 surfaces in each clinic (Arm rest of dental chair, floor beneath ...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Publication date: Available online 5 November 2019Source: Inorganica Chimica ActaAuthor(s): Mousumi Nayak, Ashish Kumar Singh, Pradyot Prakash, Rajni Kant, Subrato BhattacharyaAbstractThiosalicylato complexes of Zn(II) and Hg(II), [Zn(SC6H4CO2)(TMEDA)]2 (1) and [TMEDA-H2][Hg(SC6H4CO2)2] (2) where (TMEDA = N,N,N',N'-tetramethylethylenediamine) have been synthesized and characterized by spectral and single crystal X-ray analyses. Thiosalicylate opted different binding modes; in 1 all three binding sites are used whereas in 2 bonding of the ligands are mainly through S atoms, however, Hg-O distances indicate the existence...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
ConclusionsAn antibiotic allergy label was associated with significantly higher rates of MRSA colonization but no statistical difference with VRE colonization.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
AbstractHistorically, vancomycin has been considered a primary therapeutic option for treating infections withStaphylococcus aureus, but isolates with reduced vancomycin susceptibility (SA-RVS) (MIC  ≥ 4 μg/mL) have emerged. Telavancin, a semisynthetic lipoglycopeptide, is an alternative treatment option forS. aureus, but data examining telavancin activity against SA-RVS are limited. In the present study, we characterize 300 isolates ofS. aureus isolates (50 vancomycin-susceptible (VSSA) isolates and 250 SA-RVS isolates) from a large tertiary care, academic medical center, 51.8% of which were methi...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
AbstractMethicillin-resistantStaphylococcus aureus (MRSA) is an important cause of infections in humans. Photodynamic therapy using blue light (450  nm) could possibly be used to reduce MRSA on different human tissue surfaces without killing the human cells. It could be less harmful than 300–400 nm light or common disinfectants. We applied blue light ± riboflavin (RF) to MRSA and keratinocytes, in an in vitro liquid layer model, and compar ed the effect to elimination using common disinfection fluids. MRSA dilutions (8 × 105/mL) in wells were exposed to blue light (450  nm) &...
Source: Lasers in Medical Science - Category: Laser Surgery Source Type: research
Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogenic bacterium that causes nosocomial and community-acquired infections. Annual report of nationwide survey in Japan revealed MRSA was detected at 100% of the hospitals in 2017 (Japan Nosocomial Infections Surveillance (JANIS), 2019). Moreover, MRSA infection can cause severe illness not only in immunocompromised patients but also in healthy people. Although anti-MRSA therapeutic agents are clinically available, MRSA infection can be life threatening.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Source Type: research
Publication date: Available online 12 November 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Violet Vakunseh Bumah, Daniela Santos Masson-Meyers, Chukuka Samuel EnwemekaAbstractIn our recent study, we showed that pulsed blue light (PBL) suppresses the growth of Propionibacterium acnes more than continuous wave (CW) blue light in vitro, but it is not known that other bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), respond similarly to PBL. The high potency of PBL relative to CW blue light makes it a suitable antimicrobial for suppressing bacterial growth in biofilms as wel...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Publication date: Available online 11 November 2019Source: The Lancet Respiratory MedicineAuthor(s): Daniel J Wolter, Frankline M Onchiri, Julia Emerson, Mimi R Precit, Michael Lee, Sharon McNamara, Laura Nay, Marcella Blackledge, Ahmet Uluer, David M Orenstein, Michelle Mann, Wynton Hoover, Ronald L Gibson, Jane L Burns, Lucas R Hoffman, Daniel J Wolter, Frankline M Onchiri, Julia Emerson, Mimi R Precit, Michael LeeSummaryBackgroundStaphylococcus aureus is the bacterium cultured most often from respiratory secretions of people with cystic fibrosis. Both meticillin-susceptible S aureus and meticillin-resistant S aureus (MR...
Source: The Lancet Respiratory Medicine - Category: Respiratory Medicine Source Type: research
This study surveys the clinical relevance of the nasalStaphylococcus aureus colonization status on intensive care unit (ICU) –acquiredS.aureus infections and compares molecular characteristics of isolates from the nose and infectious sites. The 390 patients included comprised 278 non-carriers and 112 carriers. Among the carriers, 56 were decolonized with mupirocin. Decolonization was verified through a second (negative) culture. Spa typing and virulence gene profiling were performed for all isolates. Twenty sixS.aureus infections were detected in the carriage group and 20 in the non-carriage group. Eighteen of these ...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
Abstract Omadacycline is an effective therapy for community-acquired bacterial pneumonia (CABP). Given its potent activity against methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA), we sought to determine the pharmacodynamic activity and target PK/PD exposures associated with therapeutic effect in the neutropenic mouse pneumonia model against 10 MSSA/MRSA strains. The area under the concentration-time curve (AUC)/MIC associated with 1-log kill was noted at 24h ELF and plasma AUC/MIC exposures of ∼2 (ELF range
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
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