The Response of Escherichia coli to the Alkylating Agents Chloroacetaldehyde and Styrene Oxide

Publication date: Available online 7 February 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Mark M. Muenter, Ariel Aiken, Jadesola O. Akanji, Samir Baig, Sirine Bellou, Alyssa Carlson, Charles Conway, Courtney M. Cowell, Nicholas A. DeLateur, Alexis Hester, Christopher Joshi, Caitlin Kramer, Becky Leifer, Emma Nash, Macee Qi, Meghan Travers, Kelly C. Wong, Man Hu, Na Gou, Roger W. GieseAbstractDNA damage is ubiquitous and can arise from endogenous or exogenous sources. DNA-damaging alkylating agents are present in environmental toxicants as well as in cancer chemotherapy drugs and are a constant threat, which can lead to mutations or cell death. All organisms have multiple DNA repair and DNA damage tolerance pathways to resist the potentially negative effects of exposure to alkylating agents. In bacteria, many of the genes in these pathways are regulated as part of the SOS reponse or the adaptive response. In this work, we probed the cellular responses to the alkylating agents chloroacetaldehyde (CAA), which is a metabolite of 1,2-dichloroethane used to produce polyvinyl chloride, and styrene oxide (SO), a major metabolite of styrene used in the production of polystyrene and other polymers. Vinyl chloride and styrene are produced on an industrial scale of billions of kilograms annually and thus have a high potential for environmental exposure. To identify stress response genes in E. coli that are responsible for tolerance to the reac...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research