Systematic screening and characterization of prototype constituents and metabolites of triterpenoid saponins of Caulopphyllum Robustum Maxim using UPLC-LTQ Orbitrap MS after oral administration in rats

Publication date: Available online 6 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Shaowa Lü, Shiyu Zhao, Min Zhao, Yuyan Guo, Guoyu Li, Bingyou Yang, Qiuhong Wang, Haixue KuangABSTRACTTriterpenoid saponins are the main bioactive components in Caulopphyllum Robustum Maxim (CRM), and they have been reported to have extensive pharmacological properties, such as anti-inflammatory, immunomodulatory, and anti-tumor effects. Cauloside C, Cauloside D, Leonticin D and Cauloside H are the main active chemical constituents of CRM in the treatment of rheumatoid arthritis (RA). However, their metabolic processes and products remain unclear. Therefore, the purpose of this study was to analyze the metabolic components and metabolic pathways of total saponins after oral administration of CRM effective part (CRME) in rats. In this work, we collected plasma, bile, urine and feces of rats at different sampling time points after intragastric administration. The saponins and reference substances were separated from CRME and analyzed via Thermo ScientificTM Ultra Performance Liquid Chromatography-Orbitrap Elite Combined High resolution Mass Spectrometry. According to the structural characteristics of the compounds in CRM, the pyrolysis behavior of various components was inferred in the negative ion mode. Twenty-two components were found in rat plasma, bile, urine and stool; among these components, there were 8 prototypes and 14 metabolites. Seven prototypes...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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