Functional equivalency in human somatic cell nuclear transfer ‐ derived endothelial cells

This study demonstrates the cellular function of differentiated hNT‐ESCs in an ischemic mice model in comparison to that of IVF‐ESCs derived from the embryo of a donor. The derived hN T‐ESC‐ECs were isolated by sorting for CD31+ expressing cells which exhibited signature endothelial characteristics in vitro such as tubule formation and ac‐LDL uptake. While the hNT‐ESC‐ECs possessed angiogenic potential, its impact on restoring blood perfusion to an ischemic limb was sli ghtly lower than hESC‐EC transplantation.© AlphaMed Press 2019
Source: Stem Cells - Category: Stem Cells Authors: Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research

Related Links:

AbstractLung transplantation is currently the only curative treatment for patients with end-stage lung disease; however, donor organ shortage and the need for intense immunosuppression limit its broad clinical application. Bioartificial lungs created by combining native matrix scaffolds with patient-derived cells might overcome these problems. Decellularization involves stripping away cells while leaving behind the extracellular matrix scaffold. Cadaveric lungs are decellularized by detergent perfusion, and histologic examination confirms the absence of cellular components but the preservation of matrix proteins. The resul...
Source: Surgery Today - Category: Surgery Source Type: research
Authors: Bral M, Shapiro AMJ Abstract Recent years have demonstrated a surging interest in normothermic ex situ liver perfusion, with iterative experimental and clinical studies establishing this technology as providing obvious advantages over static cold storage. In particular, the safe prolongation of liver graft preservation even up to 1 week opens up possibilities of 'on circuit' interventions, which may radically change the logistics and scope of liver transplant practice. Such approaches are rife with potential, and have yet to be fully explored. Possibilities may include, but are not limited to mitochon...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
In this study, we intravenously administrated the young mitochondria into aged mice to evaluate whether energy production increase in aged tissues or age-related behaviors improved after the mitochondrial transplantation. The results showed that heterozygous mitochondrial DNA of both aged and young mouse coexisted in tissues of aged mice after mitochondrial administration, and meanwhile, ATP content in tissues increased while reactive oxygen species (ROS) level reduced. Besides, the mitotherapy significantly improved cognitive and motor performance of aged mice. Our study, at the first report in aged animals, not only prov...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
AbstractObjective: To investigate the alteration of delayed memory and its relationship with neurogenesis and angiogenesis in vascular dementia (VD) rats after moxibustion therapy.Methods: Two hundred adult male SPF Wistar rats were chosen for the experiment. Thirty-six rats were randomly selected as the sham operation group. Except for rats in the sham operation group (n=36), the others were made into VD models by bilateral common carotid arteries occlusion (BCCAo). After modeling, the 108 survived rats were randomly divided into 3 groups: a model group, a neural stem cells (NSCs) plus endothelial progenitor cells (EPCs) ...
Source: Journal of Acupuncture and Tuina Science - Category: Complementary Medicine Source Type: research
CONCLUSIONS: ASC sheets prevented cardiac dysfunctions and remodeling after MI in a rat model via VEGF secretion. Inhibition of VEGF effects by carvedilol abolished their beneficial effects. PMID: 31527337 [PubMed - as supplied by publisher]
Source: Circulation Journal - Category: Cardiology Authors: Tags: Circ J Source Type: research
Purpose of review It has been increasingly common to use adipose tissue for regenerative and reconstructive purposes. Applications of autologous fat transfer and different stem cell therapies have significant limitations and adipose tissue engineering may have the potential to be an important strategy in the reconstruction of large tissue defects. A better understanding of adipogenesis will help to develop strategies to make adipose tissue more effective for repairing volumetric defects. Recent findings We provide an overview of the current applications of adipose tissue transfer and cellular therapy methods for soft ...
Source: Current Opinion in Organ Transplantation - Category: Surgery Tags: ORGANOGENESIS: Edited by Giuseppe Orlando Source Type: research
Background: Stem cell therapy of acute myocardial infarction is proving to be a promising approach to repair the injured myocardia. However, the time window for stem cell transplantation is important and yet difficult to determine since it produces much different therapeutic effect at different times after myocardial infarction. Stromal cell-derived factor-1 (SDF-1) plays a pivotal role in the mobilization, homing, proliferation and differentiation of transplanted stem cells. Here, by using of ultrasound molecular imaging via targeted microbubbles, we determined the dynamic expression of SDF-1 in a swine model of acute myo...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Conclusion: In conclusion, this vascular graft may hold the potential to regenerate functional small diameter vessels for cardiovascular tissue repair. PMID: 31379220 [PubMed - as supplied by publisher]
Source: Connective Tissue Research - Category: Research Tags: Connect Tissue Res Source Type: research
Abstract PURPOSE OF REVIEW: It has been increasingly common to use adipose tissue for regenerative and reconstructive purposes. Applications of autologous fat transfer and different stem cell therapies have significant limitations and adipose tissue engineering may have the potential to be an important strategy in the reconstruction of large tissue defects. A better understanding of adipogenesis will help to develop strategies to make adipose tissue more effective for repairing volumetric defects. RECENT FINDINGS: We provide an overview of the current applications of adipose tissue transfer and cellular thera...
Source: Current Opinion in Organ Transplantation - Category: Transplant Surgery Authors: Tags: Curr Opin Organ Transplant Source Type: research
The risk associated with bloodless autologous hematopoietic cell transplantation (aHCT) includes severe bleeding and profound anemia. Support with platelet (PLT) or red blood cell (RBC) transfusions are often required to prevent complications associated with temporary marrow aplasia before hematopoietic recovery. Without transfusion support, profound anemia may lead to decreased systemic perfusion and cause multi-organ failure. Similarly, profuse bleeding occurring during severe thrombocytopenia may be detrimental.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
More News: Perfusion | Stem Cell Therapy | Stem Cells | Study | Transplants