Fluorescence-enhanced p19 proteins-conjugated single quantum dot with multiplex antenna for one-step, specific and sensitive miRNAs detection.

Fluorescence-enhanced p19 proteins-conjugated single quantum dot with multiplex antenna for one-step, specific and sensitive miRNAs detection. Anal Chim Acta. 2019 Apr 11;1053:114-121 Authors: Ren X, Xue Q, Wen L, Li X, Wang H Abstract Ideal methods for miRNA detection should be rapid and amplification-free in the mix-and-measure format. Here we report a novel FRET strategy based on fluorescence-enhanced p19 proteins-conjugated single quantum dot (QD) with multiplex antenna for one-step, specific and sensitive miRNAs quantitative analysis. It is simple in design and operation, and possesses high sensitivity and selectivity. In the strategy, RNA-binding viral p19 proteins-conjugated quantum dot (p19-QD) with high fluorescence and multiplex antenna was designed, and exploited for the capture recognition element and the donor. In the presence of target miRNAs, the p19-QD would tightly capture the miRNA-21-antimiRNA-21 duplex (dsRNA) formed in solution by hybridization of the specific antimiRNA-21-Cy3 probe (receptor) to the single-stranded miRNA-21 target. The FRET detection system between QD and Cy3 was constructed, and the signal read-out was measured. Conversely, in the absence of target miRNA-21, the p19-QD refuse to capture the free single-stranded antimiRNA-21-Cy3 probe (receptor), the FRET between quantum dot and Cy3 is deterred, thereby providing a low-background signal to improve sensitivity. Benefiting from the high affinity a...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research