Mustard seeds derived fluorescent carbon quantum dots and their peroxidase-like activity for colorimetric detection of H2O2 and ascorbic acid in a real sample.

Mustard seeds derived fluorescent carbon quantum dots and their peroxidase-like activity for colorimetric detection of H2O2 and ascorbic acid in a real sample. Anal Chim Acta. 2019 Apr 25;1054:145-156 Authors: Chandra S, Singh VK, Yadav PK, Bano D, Kumar V, Pandey VK, Talat M, Hasan SH Abstract Herein, we were synthesized fluorescent carbon quantum dots via facile one-step hydrothermal treatment of mustard seeds (M-CQDs). It showed excellent optical property with fluorescent quantum yield 4.6%. The as-prepared M-CQDs exhibited peroxidase-like mimetic activity and catalyzed the oxidation of chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction mixture with the prominent peak at 652 nm. Furthermore, the peroxidase-like catalytic performance of M-CQDs followed the steady-state kinetics behavior and exhibited similar catalytic activity as that of natural enzyme Horseradish peroxidase (HRP). In addition to this, the double reciprocal plot showed a parallel line which suggested the occurrence of Ping-Pong type of mechanism. The H2O2 dependent oxidation of TMB was helpful for the colorimetric detection of H2O2 in the linear range of 0.02-0.20 mM with the limit of detection (LOD) of 0.015 mM. Interestingly, the oxidized TMB (ox-TMB) was further reduced to native TMB by the reducing agent ascorbic acid. Hence M-CQDs showed its potential towards the selective and sensitive det...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research